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Residual catalytic activity (v/vo) as a function of MT-DADMe-ImmA titration of SaMTAN. Symbols v and v0 are reaction rates in the presence and absence of inhibitor, respectively. Approximately 80% inhibition occurs when the first site is filled, and complete inhibition occurs as the second site is filled. The abscissa is the ratio of MT-DADMe-ImmA to SaMTAN dimer. The line is a linear fit to the experimental points as the MT-DADMe-ImmA/SaMTAN ratio increases to 1.0.
