Figure 3. The ability of LKB1 to suppress Yap dependent transcription and cell growth is partially reversed by phosphorylation-deficient Yap mutants.

A) HeLa cells were transfected with Yap- wild type (WT), S127A, or 5SA in combination with vector control or LKB1. TEAD dependent transcription was measured as described in Figure 1c.

B) HeLa cells were co-infected with LKB1 and vector control (◇), Yap^WT (■), Yap^S127A (▲), and Yap^5SA (X) mutants. Following puromycin selection, cell accumulation was measured as in Figure 1b.

Error and significance are represented as described in Figure 1.

C) HeLa cells stably expressing the indicated cDNAs were imaged after 10 days culture in Matrigel. Representative images are shown. Insets are 2-fold increase magnification of colonies.