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In vitro validation of knockdown by siRNAs targeting specific regions of the mRNA for MOP-r. Control and MOP-r-specific siRNAs were co-transfected with psiCHECK Oprm1 plasmid into HEK293 cells. Luciferase activities were measured and results are shown as ratios of renilla/firefly luciferase (RL/FL) activity normalized to control siRNA transfection (in %), indicating effect of knockdown by specific siRNAs.
