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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Dec 5;34(2):338–345. doi: 10.1161/ATVBAHA.113.302785

Fig. 5. Apelin and Vegfc signaling are not redundant but functionally synergize to facilitate lymphatic development in zebrafish.

Fig. 5

(A) Apelin and Vegfc signaling may synergistically promote lymphatic development. 4dpf embryos co-injected with low doses of apln and vegfc MOs contained a substantially decreased number of LECs (arrows) compared to control or single MO injected embryos. (B) Quantification of the number of LECs. (C) Apelin signaling may have a non-redundant role with Vegfc signaling during LEC development. Over-expression of Apelin by mRNA injection did not alleviate lymphatic defects in 4dpf vegfc MO-injected embryos. (D) Quantification of the number of LECs. (E) Representative images for the lymphatic vessel phenotype in 4dpf Tg(fli1a:nEGFP);Tg(kdrl:mCherry) embryos injected with apln and vegfc MOs and vegfc mRNA. vegfc mRNA injection at 1–2 cell stage only rescues the defects caused by Vegfc knockdown, but not Apln knock-down. (F) Quantification of LECs. DA, dorsal aorta; CV, cardinal vein. Scale bars are 50μm.