TABLE I.
Sample concentration (μM) | Dilution factor | Input concentration (fM) | Fraction of none-fluorescent droplets | λmes | Measured enzyme concentration in droplet (Cmes, fM) | RSD % | Measured sample concentration μM) | Average of measured sample concentration (μM) | |
---|---|---|---|---|---|---|---|---|---|
0.89 0.26 | 4.50 × 10−8 | 40 | 0.899 | 0.106 | 42.0 | 6.8 | 0.93 | 0.880.02 | |
1.13 × 10−7 | 101 | 0.7820.010 | 0.2460.013 | 97.4 | 5.6 | 0.86 | |||
2.25 × 10−7 | 200 | 0.6160.007 | 0.4850.011 | 192 | 2.9 | 0.850.02 | |||
3.38 × 10−7 | 301 | 0.4720.011 | 0.7510.023 | 297 | 3.5 | 0.880.03 | |||
4.50 × 10−7 | 401 | 0.3660.006 | 1.0050.016 | 398 | 2.4 | 0.880.02 | |||
9.00 × 10−7 | 801 | 0.1180.006 | 2.1430.051 | 848 | 3.0 | 0.940.03 | |||
1.35 × 10−6 | 1202 | 0.0550.006 | 2.913 | 115047.8 | 4.2 | 0.850.04 |