Figure 7.

Quantitative real-time PCR analysis of select targets regulated by β-D-glucan in MCF-7 cells. Cells were grown in phenol red-free IMEM + 5% DCC for 48 h prior to addition of DMSO (vehicle control), 10 nM E₂, 100 nM 4-OHT or the indicated concentrations of DMSO-dissolved β-D-glucan for 24 h. qPCR for each target gene was normalized to 18S rRNA and values were compared to fold expression in vehicle (DMSO)-treated MCF-7 cells. Values are the average of triplicate determinations ± SEM within one experiment.