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. 2014 Apr 7;9(4):e93172. doi: 10.1371/journal.pone.0093172

Figure 5. Biochemical analysis of the ECM content of the vaginal wall of virgin, parous and pregnant sheep.

Figure 5

A.% total collagen per dry weight assessed by hydroxyproline assay in the anterior (black bars) and posterior (grey bars) vaginal wall. B. % collagen III/(I+III) analyzed by SDS-PAGE. C. % ETAP per dry weight by Amino Acid Analysis. D. % GAG per dry weight by DMMB assay. Data is presented as mean (±SEM), n = 6/group for parous and pregnant and n = 3 for virgin ewes. * p<0.05, *** p<0.001, **** p<0.0001.