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. 2014 Apr 7;9(4):e93840. doi: 10.1371/journal.pone.0093840

Figure 3. Increased expression of Fgf23, Dmp1, and Fam20c in Hyp bones occurred before birth.

Figure 3

(A) Serum levels of FGF23 in WT (white bars) and Hyp (black bars) male mice at E18.5 and 4 and 10 weeks of age. Regarding samples at E18.5, serum from genetically identical littermates were pooled together and assayed as one sample. Regarding samples at 4 and 10 weeks of age, data are shown as the mean ± SEM (n = 3–4). *P<0.05 vs. WT. (B) Serum Pi levels in WT (white bars) and Hyp (black bars) male mice at E18.5 and 4 and 10 weeks of age. Data are shown as the mean ± SEM (n = 3–7). *P<0.05 vs. WT. (C) Gene expression in the bones of WT (white bars) and Hyp (black bars) male mice at E18.5 and 4 weeks of age. RNA was extracted from the bones after removal of the bone marrow and surrounding soft tissue, and was used for real-time PCR to examine the expression of the indicated genes. Expression of the target cDNA was standardized to that of Gapdh. Data are shown as the mean ± SEM. n = 5–9 in E18.5 fetuses. n = 3 in 4-week-old mice. *P<0.05 vs. WT.