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. 2014 Apr 7;9(4):e93840. doi: 10.1371/journal.pone.0093840

Figure 4. Expression of Sost and Phex was retained after 48-hour collagen-embedded culture of the isolated osteocytic cells.

Figure 4

The fresh cells of osteocyte-rich Fractions 6–9 isolated from 10-week-old WT bones were aliquoted, and RNA was extracted immediately (0 h) or after 48-hour collagen-embedded culture (48 h). Real-time PCR was performed to examine the expression of the osteoblastic gene Kera (A) and osteocytic genes Sost (B) and Phex (C). The copy number of the target cDNA in each sample was estimated by referring to a standard curve, which was standardized to that of Gapdh. Data are shown as the mean ± SEM of 3 isolations. *p<0.05 vs. 0 h.