Table 3. Nasal polyp tissue (n = 9) was incubated with FF and MF at different concentrations for 1 hour, and then washed and SEB was added immediately (0 hour), after 6 or 24 hours (pre-challenge incubation with time interval).
| Cytokine | Fluticasone furoate | Mometasone furoate | ||||||
| time | Max. inhibition (median (Interquart. range) in % | Concentr. of max. inhibition | Lowest concentr. with significant inhibition | p-value for max. inhibition | Max. inhibition (median (Interquart. range) in % | Concentr. of max. inhibition | Lowest concentr. with significant inhibition | |
| IFN-γ | 0 h | 63 (58.3–78) | 10−8 M | 10−11 M | P = 0.0054 | 34 (9,5–43.5) | 10−8 M | 10−8 M |
| 6 h | 86 (82.5–90) | 10−8 M | 10−11 M | P = 0.007 | 71 (36.8–81.3) | 10−9 M | 10−9 M | |
| 24 h | 94 (91.3–96) | 10−9 M | 10−11 M | P = 0.0033 | 70 (55.5–73.5) | 10−8 M | 10−9 M | |
| IL-2 | 0 h | 65 (50–73.8) | 10−8 M | 10−11 M | P = 0.0092 | 39 (13.5–43.8) | 10−9 M | 10−9 M |
| 6 h | 90 (79.591.5) | 10−9 M | 10−11 M | P = 0.0041 | 74 (37.5–76.3) | 10−9 M | 10−11 M | |
| 24 h | 93 (87.5–95) | 10−10 M | 10−11 M | P = 0.0297 | 64 (60.8–86) | 10−8 M | 10−11 M | |
| IL-5 | 0 h | 90 (80.8–94.3) | 10−8 M | 10−11 M | P = 0.0118 | 51 (27.8–73.3) | 10−8 M | 10−8 M |
| 6 h | 90 (81.5–97.3) | 10−9 M | 10−11 M | P = 0.1022 | 76 (63.5–90.3) | 10−9 M | 10−9 M | |
| 24 h | 85 (84.3–95.8) | 10−11 M | 10−11 M | P = 0.1599 | 82 (67.5–92.5) | 10−8 M | 10−11 M | |
| IL-17 | 0 h | 78 (72.3–85) | 10−8 M | 10−11 M | P = 0.0027 | 47 (28.5–70.3) | 10−8 M | 10−8 M |
| 6 h | 83 (77.8–92.3) | 10−8 M | 10−11 M | P = 0.038 | 72 (49–79.5) | 10−9 M | 10−11 M | |
| 24 h | 90 (84.8–94.8) | 10−10 M | 10−11 M | P = 0.0181 | 70 (60.8–79.5) | 10−8 M | 10−10 M | |
| TNF-α | 0 h | 70 (64–78) | 10−11 M | 10−11 M | P = 0.0054 | 38 (25.3–54.5) | N | N |
| 6 h | 70 (58.3–76) | 10−11 M | 10−11 M | P = 0.112 | 63 (23–66.3) | 10−9 M | 10−10 M | |
| 24 h | 74 (60.5–81.8) | 10−10 M | 10−11 M | P = 0.2248 | 61 (55.3–69.5) | 10−8 M | 10−11 M | |
Cells were then incubated for another 24 hours. When SEB stimulation was performed immediately, FF demonstrated a faster onset of action with a significantly higher maximal inhibition of all cytokines vs. MF. For most cytokines and time points, FF reached a significant reduction at a lower concentration; however, when the time interval between pre-incubation and challenge was minimally 6 hours, the suppression capacity of MF reached the same levels as FF for IL-5 and TNF-α.
N = no significant reduction reached at any concentration tested.