Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 27;4:16. doi: 10.1186/2045-3701-4-16

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Cook et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Co-localization of autophagosomes and cellular organelles. A. LCC9 cells were transfected with GFP-LC3 for 24 hours. Cells were incubated with either endoplasmic reticulum dye, Golgi complex-RFP, or MitoTracker-RFP and treated with 500 nM ICI to induce autophagy. Confocal microscopy was used to determine the co-localization of LC3 puncta with different cellular organelles. B. The percent of LC3-positive autophagosome co-localizing with different cellular structures was determined. n = 5-6.