Figure 2. The evolutionary origin of diaphanous. (A) Phylogenetic tree based on the RBD/FH3 region and the FH2 domain. Green circles denote an approximate Likelihood Ratio (aLRT) support of > 0.8, a black circle indicates aLRT > 0.9. For accession numbers of Dia sequences see Supplemental Materials. (B) Differentially conserved sites between vertebrate Dia paralogs. Positions with a zscore > 5 were mapped onto the structure of mDia1 (pdb: 3EG5; mDia1 in green, interacting Rho GTPase in blue). Sites conserved between Dia2 and Dia3 in yellow, differing sites in orange.