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. 2014 Apr 7;9(4):e93563. doi: 10.1371/journal.pone.0093563

Figure 8. PD123319 and AG490 block the stimulatory effect of Ang II on Pax-2 expression in NRK-52E cells.

Figure 8

NRK-52E cells were pretreated with PD123319 (10−6 mol/L) and AG490 (10−6 mol/L) in serum-free medium for 10 min before Ang II (10−9 mol/L) administration. Cells were harvest at 1 h (for RT-PCR analysis) and 2 h (for western blot analysis) post exposure. RT-PCR (A) and western blot (B) results showed that both AT2R blocker PD123319 and JAK2 blocker AG490 could block the stimulatory effect of Ang II on Pax2 expression in NRK-52E cells (*P<0.05).