Table 1. Tentative assignments of Raman bands (human tissue).
Position of character peak | Biochemical Assignments | Biomolecular Assignments |
622 | δc-c (Twisted) phenylalanine | Phenylalanine, Tyrosine |
645 | dC | Nucleic acid |
669 | dT | Nucleic acid |
721 | dA | Nucleic acid |
758 | νs (Indole ring breathing) | Tryptophan |
786 | νsPO2- group | DNA, RNA |
829 | νasPO2- | Nucleic acid |
855 | ν C-C Proline | Protein (collagen) |
877 | ν C-C Hydroxyproline | Protein (collagen) |
938 | ν C-C Proline and valine (α-helix) | Protein (collagen) |
957 | δCH3 (deformed) | Lipid, protein |
1001–1004 | ν ring breathing | Phenylalanine, protein |
1033 | δC-H (Plane bending) aromatic compound | Phenylalanine, tryptophan, tyrosine |
1065 | ν -C = C = C- | |
1083–1095 | νs -O-P-O-, v C-C phospholipid | Nucleic acid (DNA, RNA) |
1127 | ν C-N protein, v C-C lipid | Protein, Lipid |
1157.00 | Polyene chain | Carotenoids |
1173 | δC-H (In-plane bending) phenylalanine, tyrosine | Phenylalanine, tyrosine (protein) |
1209 | ν C-C6H5 Tryptophan and phenylalanine | Tryptophan, phenylalanine (protein) |
1230–1240 | Amino compound III (β fold) | |
1245–1255 | Amino compound III (random coil, corner) | |
1264–1272 | Amino compound III (α-helix) | |
1266 | ν C-H and δH-N- (Bending) amino compound III | Protein |
1288–1304 | Lipid ch2 bending vibration and bending vibration ch2ch3 | |
1320–1340 | ν Ch2ch3 and δCh2ch3 (Swing) proteins and nucleic acids | Protein, nucleic acid |
δC-H (Plane deformation) ordinary olefin | Unsaturated fatty acid | |
1448 | δCH2 (Bending) proteins and lipids | Protein, Lipid |
1527 | ν C-C Carotenoids | Carotenoid |
1551 | νas -NO2 | |
1585 | ν c = c Lipid | Unsaturated fatty acid |
1605 | ν C = C Aromatic compound | Phenylalanine, tyrosine |
1617 | ν C = C | Porphyrin and tryptophan |
1640-1680 | Amino compounds I, α helix | Protein |
ν: stretching vibration, νas: asymmetric stretching vibration, νs: symmetric stretching vibration, δ: bending, deformed, swing (relative peak intensity = the peak intensity/average intensity of the full spectrum).