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. 2014 Apr 7;9(4):e94038. doi: 10.1371/journal.pone.0094038

Figure 4. Localisation of Crb in BCs is independent from its known protein binding motifs.

Figure 4

(A) Schematic representation of the Crb protein and its variants used in this study. Green rectangles: EGF-like repeats, brown hexagons: laminin A G like domains, grey bar: transmembrane domain (TM). (B) Amino acid sequences of the cytoplasmic tails of wild-type and mutant Crb proteins used in this study. Blue: FERM domain-binding motif, brown: PDZ domain-binding motif. Red: point mutations. (C–D″) Confocal microscopy images of cross sections through the large intestine of stage 15 homozygous crb8F105 (C–C″) and foscrbY10A,ΔERLI (D–D″) embryos stained with anti-Crb (magenta), anti-α-Spectrin (grey) and anti-Sas (green). Crb is upregulated and apically localised in the BCs (white arrowheads), but is not detectable in the PCs.