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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Feb 5;69:43–51. doi: 10.1016/j.yjmcc.2014.01.017

Figure 7. Enhancing proteasome function by muscarinic stimulation is PKG-dependent in cardiomyocytes.

Figure 7

Cultured NRVMs were infected with Ad-GFPu (10 MOI) and cultured for additional 24 hours before being treated with pilocarpine (Pilo, 10μM), KT5823 (KT, 1μM), Pilo plus KT (Pilo+KT; P+K), or vehicle control (saline) for 24h. A and B, Representative images (A) and the pooled densitometry data (B) of western blot analyses for the indicated proteins. C and D, Representative images (C) and the pooled densitometry data (D) of western blot analysis for GFPu. α-Actinin was probed as loading control. E, Changes in the proteasomal chymotrypsin-like, caspase-like, and trypsin-like peptidase activities in NRVMs. *p<0.05, #p<0.01 vs. the saline control group; n=6 repeats.