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. 2014 Apr 7;9(4):e93991. doi: 10.1371/journal.pone.0093991

Figure 4. Telomere length after recovery from WRN siRNA transfection.

Figure 4

After 54 PD of continuous WRN knockdown (after telomeres had shortened by almost 50% of the original length), WRN siRNA transfections of a subset of HeLa and VA-13 cells were ceased and cells allowed to recover in culture for an additional 54 PD. Average relative telomere repeat lengths were then measured by qRT-PCR. Results are graphically represented as the difference between a telomere-specific PCR reaction in comparison to the single copy gene albumin PCR reaction as delta Ct.