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. 2014 Apr 7;9(4):e93991. doi: 10.1371/journal.pone.0093991

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© 2014 Gocha et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Telomerase-positive HeLa cells and three ALT cell lines, VA-13, Saos-2 and U-2 OS, were transfected with SC or WRN siRNAs and fixed 48 hours after transfection. Fixed cells were immunofluorescently labeled with antibodies to BRCA1 and PML and scored for co-localization. Percentage of cells with co-localized foci were averaged from three independent experiments.