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. 2014 Apr 7;9(4):e93991. doi: 10.1371/journal.pone.0093991

Figure 10. Telomere recombination measured by T-SCE is variably affected by WRN and/or BRCA1 knockdown.

Figure 10

ALT cell lines VA-13, Saos-2, and U-2 OS were transfected with WRN, BRCA1, or WRN/BRCA1 siRNAs and metaphase spreads were processed for T-SCE after 72 hours. Telomeres were fluorescently labeled and analyzed. A. A representative image of a metaphase spread from VA-13 cells shows DAPI-labeled chromosomes in blue and Cy-3 labeled telomeres in red. B. Results depict the frequency of T-SCE per chromosome analyzed from at least 30 metaphase spreads per treatment group. Western blots depict the ability of BRCA1 siRNAs to knock down BRCA1 in each cell line.