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. 2014 Apr 7;9(4):e94032. doi: 10.1371/journal.pone.0094032

Figure 4. Atrophic response to denervation in MuRF1-hiLUCs reporter rats vs. the wild-type littermates.

Figure 4

(A) Extent of muscle atrophy in denervated vs. sham-operated rats after 14 days of denervation. Hindlimb muscles were harvested on day 14 after surgeries from denervated (n = 8) or sham operated (n = 4) MuRF1-hiLUCs reporter rats and their wild-type littermates that underwent denervation (n = 8). Muscle weights from the denervated leg were normalized to the muscle weights from the contralateral leg of the same rat, since no compensatory hypertrophy was detected in the contralateral leg (Figure S6). Data are presented as means (symbols) + SEM (lines). Statistics were performed using ordinary one-way ANOVA with Tukey's multiple comparisons test. *P<0.05, ***P<0.001, ****P<0.0001. (B) Endogenous MuRF1 mRNA induction in the MURF1-hiLUCs reporter rats compared to wild-type littermates. Muscles from both hindlimbs of the sham-operated and denervated MuRF1-hiLUCs reporter rats and their denervated wild-type littermates were harvested on day 14 after the surgeries. 20∼30 mg of the gastrocnemius muscles were homogenized and subjected to the Panomics Quantigene multiplex assay. Results were normalized to the geometric mean of three house keeping genes: hprt1, gusb and hmbs. Statistics were performed using one-way ANOVA with Tukey's multiple comparisons test. *P<0.05, **P<0.01, ****P<0.0001.