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. 2014 Apr 7;9(4):e93912. doi: 10.1371/journal.pone.0093912

Table 1. Sequences of primers used in this study.

Primers Sequences Purpose in this study
LacCu1 5′-CAY TGG CAY GGN TTY TTY CA-3′ Gene fragment
LacCu2 5′-G RCT GTG GTA CCA GAA NGT NCC-3′ Gene fragment
Lac3 GSP1 5′-GGACATTGATTGACACCAGTCGCGCCA-3′ 5′-RACE of lac3
Lac4 GSP1 5′-CTCCGGAACCACCATCAGCCCAATTAG-3′ 5′-RACEof lac4
Lac3 GSP2 5′-CTCACGACCTCCCGCTTGGGAATAACCAAC-3′ 3′-RACEof lac3
Lac4 GSP2 5′-GGCGCCAATGCTTTTGCGCTTTCTGTCGG-3′ 3′-RACEof lac4
Lac3F 5′-CGGAATTCTCAGTCCTCATACCGCACTCGAC-3′ pPICZαB-Lac3,
Lac3R 5′-GCTGGCGGCCGCAACTGGCATTGGTAAAGAAGAGACA-3′ pPICZαB-Lac3,
Lac4F 5′-CGGAATTCCTTTCTGTCGGTCCACGAGCAAC-3′ pPICZαB-Lac4
Lac4R 5′-GCTGGCGGCCGCATGTTTCACCATAGGCACAAT-3′ pPICZαB-Lac4
pPICZαB-10AAF 5′-GATCTAGAAGAGTTGGTGTTGAAAGGGGGGAATGGCGTCGGAATTCCTGCAGCTTCAGCCT-3′ pPICZαB-10AA
pPICZαB-10AAR 5′-TTCCGCGGGAACAAAAACTCATCT-3′ pPICZαB-10AA
Lac310AAF 5′-GCTCTAGATCAGTCCTCATACCGCACTCGAC-3′ pPICZαB-10AALac3
Lac310AAR 5′-GTCCCCGCGGAACTGGCATTGGTAAAGAAGAGACA-3′ pPICZαB-10AALac3
Lac410AAF 5′-GCTCTAGACTTTCTGTCGGTCCACGAGCAAC-3′ pPICZαB-10AALac4
Lac410AAR 5′-GTCCCCGCGGATGTTTCACCATAGGCACAAT-3′ pPICZαB-10AALac4
Lac3mycR 5′-GTCCCCGCGGTCAATGATGATGATGATGATGAACTGGCATTGGTAAAGAAGAGACA-3′ pPICZαB-10AALac3-ΔMyc
Lac4mycR 5′-GTCCCCGCGGTCAATGATGATGATGATGATGATGTTTCACCATAGGCACAAT-3′ pPICZαB-10AALac4-ΔMyc

In the degenerate primers the following abbreviations were used Y = C, T; N = A, G, C, T; M = C, A; R = A, G; V = G, A, C.