Table 1. Sequences of primers used in this study.
Primers | Sequences | Purpose in this study |
LacCu1 | 5′-CAY TGG CAY GGN TTY TTY CA-3′ | Gene fragment |
LacCu2 | 5′-G RCT GTG GTA CCA GAA NGT NCC-3′ | Gene fragment |
Lac3 GSP1 | 5′-GGACATTGATTGACACCAGTCGCGCCA-3′ | 5′-RACE of lac3 |
Lac4 GSP1 | 5′-CTCCGGAACCACCATCAGCCCAATTAG-3′ | 5′-RACEof lac4 |
Lac3 GSP2 | 5′-CTCACGACCTCCCGCTTGGGAATAACCAAC-3′ | 3′-RACEof lac3 |
Lac4 GSP2 | 5′-GGCGCCAATGCTTTTGCGCTTTCTGTCGG-3′ | 3′-RACEof lac4 |
Lac3F | 5′-CGGAATTCTCAGTCCTCATACCGCACTCGAC-3′ | pPICZαB-Lac3, |
Lac3R | 5′-GCTGGCGGCCGCAACTGGCATTGGTAAAGAAGAGACA-3′ | pPICZαB-Lac3, |
Lac4F | 5′-CGGAATTCCTTTCTGTCGGTCCACGAGCAAC-3′ | pPICZαB-Lac4 |
Lac4R | 5′-GCTGGCGGCCGCATGTTTCACCATAGGCACAAT-3′ | pPICZαB-Lac4 |
pPICZαB-10AAF | 5′-GATCTAGAAGAGTTGGTGTTGAAAGGGGGGAATGGCGTCGGAATTCCTGCAGCTTCAGCCT-3′ | pPICZαB-10AA |
pPICZαB-10AAR | 5′-TTCCGCGGGAACAAAAACTCATCT-3′ | pPICZαB-10AA |
Lac310AAF | 5′-GCTCTAGATCAGTCCTCATACCGCACTCGAC-3′ | pPICZαB-10AALac3 |
Lac310AAR | 5′-GTCCCCGCGGAACTGGCATTGGTAAAGAAGAGACA-3′ | pPICZαB-10AALac3 |
Lac410AAF | 5′-GCTCTAGACTTTCTGTCGGTCCACGAGCAAC-3′ | pPICZαB-10AALac4 |
Lac410AAR | 5′-GTCCCCGCGGATGTTTCACCATAGGCACAAT-3′ | pPICZαB-10AALac4 |
Lac3mycR | 5′-GTCCCCGCGGTCAATGATGATGATGATGATGAACTGGCATTGGTAAAGAAGAGACA-3′ | pPICZαB-10AALac3-ΔMyc |
Lac4mycR | 5′-GTCCCCGCGGTCAATGATGATGATGATGATGATGTTTCACCATAGGCACAAT-3′ | pPICZαB-10AALac4-ΔMyc |
In the degenerate primers the following abbreviations were used Y = C, T; N = A, G, C, T; M = C, A; R = A, G; V = G, A, C.