Table 2. Real time PCR Primers and Annealing Temperatures.
PCR Primers | Forward (5′-3′) | Reverse (5′-3′) | Annealing Temp (°C) |
HPRT | GTAATGATCAGTCAACGGGGGAC | CCAGCAAGCTTGCAACCTTAACCA | 60 |
CXCL9 | ACTCGGCAAATGTGAAGAAG | CCCATTAAGATTCAGGGTGC | 58 |
IFN-γ | GCCATCAGCAACAACATAAGC | TGGGACAATCTCTTCCCCAC | 58 |
IL-4 | ATGGGTCTCAACCCCCAGCTAGT | GCTCTTTAGGCTTTCCAGGAAGTC | 57 |
IL-10 | CGGGAAGACAATAACTG | CATTTCCGATAAGGCTTGG | 58 |
IL-12/23 p40 | ACTCACATCTGCTGCTCCAC | GGGAACTGCTACTGCTCTTGA | 60 |
IL-17a | CAGAAGGCCCTCAGACTACC | CTTTCCCTCCGCATTGACAC | 56.6 |
IL-17f | ACTTGCCATTCTGAGGGAGG | TGATGCAGCCTGAGTGTCTG | 57.1 |
IL-23 p19 | CCAGCGGGACATATGAATCTAC | GATGTCAGAGTCAAGCAGGTG | 55.3 |