Table 2. Real time PCR Primers and Annealing Temperatures.

PCR Primers	Forward (5′-3′)	Reverse (5′-3′)	Annealing Temp (°C)
HPRT	GTAATGATCAGTCAACGGGGGAC	CCAGCAAGCTTGCAACCTTAACCA	60
CXCL9	ACTCGGCAAATGTGAAGAAG	CCCATTAAGATTCAGGGTGC	58
IFN-γ	GCCATCAGCAACAACATAAGC	TGGGACAATCTCTTCCCCAC	58
IL-4	ATGGGTCTCAACCCCCAGCTAGT	GCTCTTTAGGCTTTCCAGGAAGTC	57
IL-10	CGGGAAGACAATAACTG	CATTTCCGATAAGGCTTGG	58
IL-12/23 p40	ACTCACATCTGCTGCTCCAC	GGGAACTGCTACTGCTCTTGA	60
IL-17a	CAGAAGGCCCTCAGACTACC	CTTTCCCTCCGCATTGACAC	56.6
IL-17f	ACTTGCCATTCTGAGGGAGG	TGATGCAGCCTGAGTGTCTG	57.1
IL-23 p19	CCAGCGGGACATATGAATCTAC	GATGTCAGAGTCAAGCAGGTG	55.3