Table 1. Summary of transit peptides and targeting sequences used in this study.

Vector	Location	Transit sequence	Size	Function	Reference
pBR28	Nuclear	2x SV40 NLS	20 aa	Tandem copy of a nuclear localization sequence from SV40	commonly used in mammalian vectors
pBR29	Mitochondria	atpA	45aa	Alpha subunit of mitochondrial ATP synthase.	this report
pBR30	ER	BIP1	31aa	Chaperone, Hsp70 superfamily.	this report
pBR31	ER	ARS1	30aa	Periplasmic protein involved in mineralization of sulfate by hydrolyzing sulfate esters.	Rasala et al., 2012
pBR32	Chloroplast	PSAD	35aa	Protein of Photosystem I.	Fischer and Rochaix, 2001