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. 2014 Apr 7;9(4):e93921. doi: 10.1371/journal.pone.0093921

Figure 5. RL2 treatment induces autophagic changes in MDA-MB-231 cells.

Figure 5

A. Cells were treated with RL2 (0.2 mg/mL) or non-treated (control) for 8 h and fixed with 4% paraformaldehyde followed by immunostaining with anti-LC3A/B primary and FITC-conjugated secondary antibody. Images are representative of two independent experiments. B. MDA-MB-231 cells were treated with RL2 (0.2 mg/mL) for various time points (0, 2, 6 and 24 h). Total lysates were prepared and subjected to Western blot analysis. Shown are representative blots of five independent experiments.