Table 2. Overall parameters calculated from SAXSa.
Sample | c, mg/ml | R g, nm | D max, nm | V p, nm3 | MMexp, kDa | χab | χrb | χeom |
M.NlaX | 1.6–7.0 | 2.36±0.04 | 7.0±0.5 | 68±7 | 33±4 | 1.12 | 1.21b | – |
M.SsoII | 1.0–3.3 | 3.01±0.04 | 11.0±0.5 | 77±8 | 38±5 | 1.25 | 1.46 | 1.03 |
M.SsoII–DNA | 1.0–1.5 | 2.79±0.04 | 11.0±0.5 | 85±10 | 45±6 | 1.10 | 1.78 | 1.05 |
Notations: R g, radius of gyration; D max, maximum size of the particle; V p, excluded volume of the hydrated particle; MMexp, experimental molecular mass of the solute; χab, χrb and χeom, values for the fit from ab initio models, from rigid body modeling using BUNCH and from EOM, respectively.
in case of M.NlaX, χrb corresponds to the fit from the crystallographic structure of M.HhaI.