Figure 5.

Enhanced allogenetic T cell proliferation stimulated by RBcs-exposed DCs. After treatment with TNF-α (A) or LPS (B) for 24 h, varying numbers (400, 2000, or 10000) of irradiated (30 Gy) DCs were added to triplicate wells containing 2 × 10⁵ purified allogeneic T lymphocytes and incubated for 4 days. Cultures were pulsed during the final 8 h of incubation, and incorporation of [³H]-thymidine was measured. The data are mean values ± SD of triplicate determinations. Data shown are a representative experiment of three. *P < 0.05, vs control DCs. **P < 0.01, vs control DCs. Y79 DC: RBcs-exposed DCs