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Suilysin-induced changes in the organization of the actin cytoskeleton were dependent on the concentrations of cholesterol and GTPase. hBMEC cells were treated with sublytic concentrations of suilysin protein for indicated periods after pretreatment regimens. Actin filaments were stained with FITC-labeled phalloidin. Chol: cholesterol. Y27632: ROCK inhibitor. MβCD: cellular cholesterol depletion
