Figure 3.
BRAF-V600E localizes to circulating myeloid DCs and monocytes in peripheral blood. (A) Sorting strategy for peripheral blood mononuclear cells into monocyte (CD14+), myeloid DC (lineage− HLADR+ CD11c+ BDCA2+), and plasmacytoid DC (lineage− HLADR+ BDCA2+) fractions. (B) Percentage of BRAF-V600E cells in circulating monocyte and DC fractions from the indicated LCH patients. (Every bar represents a single sorted blood sample; technical duplicates were used in all experiments.)