Figure 4.

Crtam^−/− CD4⁺ T cells have normal TH1 and TH17 polarization in vitro. 2 × 10⁵ purified CD4⁺ T cells from LNs of WT or Crtam^−/− mice were stimulated in vitro under TH1 or TH17 polarizing conditions for 5 d (1°). For restimulation, T cells were recovered and 2 × 10⁵ cells were plated for 2 additional days without polarizing cytokines (2°). IFN-γ and IL-17 production was assessed by intracellular staining: (A and D) representative plots; (B and E) quantification; and (C and F) cytometric bead array of culture supernatants. (G) WT and Crtam^−/− mice were orally infected with 2 × 10⁹ C. rodentium and splenic titers analyzed at days 8 and 16. The CFUs detected in WT at day 16 are insignificant. Data are representative of three (A and B) or two (C) independent experiments. Statistical analysis was performed using Student’s t test (ND, not detected). Error bars are SD.