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. 2014 Apr 7;211(4):685–700. doi: 10.1084/jem.20131343

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© 2014 Willinger et al.

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Figure 5. — Effect of dynamin 2 deficiency on thymocyte chemokine receptors. (A) CCR7 and CXCR4 surface expression on mature CD4 SP thymocytes from Dnm2 HET and KO mice (n = 6). (B) Chemotaxis of mature CD4 SP thymocytes from Dnm2 HET and KO mice. Migration through 5-µM Transwells in response to 0.3 µg/ml CXCL12 or 1 µg/ml CCL21 was assessed by flow cytometry after 3 h (n = 5–9). (C) Mixed Dnm2 HET/B6 and Dnm2 KO/B6 bone marrow chimeras generated as in Fig. 1 G were treated with saline or 1 mg AMD3100. After 24 h of continuous treatment, CXCR4 surface expression on mature CD4 SP thymocytes was measured by flow cytometry. (A and C) Green histograms show isotype staining (backgrd). (D) Number of egressing WT (B6) CD4 SP thymocytes from mixed bone marrow chimeras treated with saline (n = 9) or AMD3100 (n = 10) as in C. (E) Ratio of egressing CD4 SP thymocytes from Dnm2 HET/B6 and Dnm2 KO/B6 mixed bone marrow chimeras treated with saline or AMD3100 (n = 5) as in C. The dashed line indicates a control cell/KO cell ratio of 1. All error bars represent SEM. *, P < 0.05; **, P < 0.01 by unpaired Student’s t test (B) or one-way ANOVA, Tukey’s post-test (E). Results are representative of or combined from two (A and C–E) or five (B) experiments.