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. 2014 Apr 7;211(4):685–700. doi: 10.1084/jem.20131343

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© 2014 Willinger et al.

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Figure 6. — Differential sensitivity of thymocyte-expressed GPCRs to ligand-induced internalization. (A and B) Thymocytes from WT mice were incubated with the indicated concentrations of S1P, CXCL12, or CCL21 in vitro. Flow cytometry was used to measure S1PR1, CXCR4, and CCR7 surface expression in response to their ligands S1P, CXCL12, and CCL21, respectively. (A) Representative histograms showing S1PR1 and CCR7 surface expression on mature CD4 SP thymocytes and CXCR4 expression on DP thymocytes. (B) Ligand-induced down-modulation of S1PR1, CXCR4, and CCR7 in response to their respective ligands (n = 6). Receptor down-modulation is shown as percent surface down-modulation (calculated as described in Materials and methods) to account for differences in staining intensity between receptors and to compare them in the same graph. Error bars represent SEM. Results are representative of or combined from two experiments.