Figure 6.

miR-134 overexpression and MET inhibition repress neurosphere formation and induce stem-cell differentiation. (a) Neurosphere formation assay in response to miR-134 transfection in GSCs. The data show that miR-134 reduces the number and size of GSC neurospheres (left panel shows photomicrographs of neurospheres; right panel shows quantification of the previous). (b) Immunoblots showing the effects of miR-134 on GSC stem-cell markers CD133 and nestin and differentiation markers GFAP and Tuj1. (c) Neurosphere formation assay in response to MET activation with HGF or inhibition with Crizotinib in GSCs. The data show that HGF induces and Crizotinib inhibits neurosphere formation (left panel shows photomicrographs of neurospheres; right panel shows quantification of the previous). (d) Immunoblots showing the effects of MET activation with HGF or inhibition with Crizotinib on GSC stem-cell and differentiation markers. (e) Neurosphere formation assay showing that siRNA-mediated knockdown of KRAS and STAT5B reduces GSC neurosphere formation (left panel shows siRNA transfection efficiency by immunoblots and right panel shows quantification of the neurosphere numbers). *P<0.05