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. 2014 Jan 31;21(5):735–747. doi: 10.1038/cdd.2013.200

Figure 5.

Figure 5

Smac mimetic decreases stem cell markers. (a) GBM10 cells were treated with 1 μM BV6 or 2% FCS for 7 days or DMSO was used as solvent. mRNA levels of nestin (NES), CD133, Sox2 and Nanog were analyzed by quantitative RT-PCR and results are presented as fold increase of DMSO-treated cells. Mean+S.E.M. of three independent experiments performed in triplicate is shown. (b and c) GBM9 cells were treated for 10 days with 0.6 μM BV6 or DMSO; GBM10 and NSCs for 7 days with 1 μM BV6 or DMSO. Cells were dissociated and stained with anti-CD133-PE antibody or with isotype control for FACS analysis. A representative experiment from three independent experiments is shown (b). The percentage of CD133-positive cells with mean+S.E.M. of three independent experiments is shown (c); *P<0.05; **P<0.01; n.s., not significant. (d) GBM9 cells were treated for 10 days with 0.6 μM BV6 or DMSO; GBM10 and NSCs for 7 days with 1 μM BV6 or DMSO. Then, cells were fixed and stained with anti-nestin antibody and counterstained with DAPI. Representative pictures of four independent experiments are shown; scale bar, 500 μm