Figure 7.

Smac mimetic reduces tumorigenicity of GBM CSLCs in vivo and increases survival of mice. (a–c) GBM9 cells were stereotactically injected after treatment with 0.6 μM BV6 or DMSO for 10 days at decreasing dilutions (a: 500 000 cells, eight mice were injected with BV6-treated cells and seven mice with DMSO-treated cells; b: 100 000 cells, eight mice per condition; c: 10 000 cells, eight mice per condition) into the corpus callosum of athymic nude mice. Overall survival curves of mice were estimated by the Kaplan–Meier method. (d) Representative H&E, GFAP, Ki67 and caspase-3 stainings of tumors derived from BV6- and DMSO-treated GBM9 cells are shown. Scale bars (500 μm) are shown for H&E stainings; GFAP, Ki67 and caspase-3 stainings correspond to sixfold magnification of the black square located on the H&E stainings. Black arrows highlight apoptotic cells; see also Table 1