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. 2014 Jan 24;21(5):797–810. doi: 10.1038/cdd.2014.3

Figure 1.

Figure 1

ISM is internalized into ECs through clathrin-dependent endocytosis and internalization is critical for its antiangiogenic/proapoptotic function. (a) Soluble but not immobilized ISM was internalized into ECs. (b) ISM was internalized into ECs in a dose-dependent manner. (c) ISM was internalized into ECs in a time-dependent manner. (d) Internalization of ISM was blocked by chlorpromazine but not nystatin in a dose-dependent manner. (e) IF detection of internalized ISM in control, nystatin or chlorpromazine pretreated HUVECs. ISM (green) internalization was only suppressed by chlorpromazine treatment. Nuclei were stained with DAPI (blue). (f) Chlorpromazine, but not nystatin, effectively reduced the anti-tube formation activity of ISM. **P<0.01, n=3. Error bars denote S.E.M. (g) Chlorpromazine, but not nystatin, significantly impaired the proapoptotic activity of ISM on ECs. *P<0.05, n=3. Error bars denote S.E.M.