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. 2014 Jan 31;21(5):774–782. doi: 10.1038/cdd.2014.6

Figure 6.

Figure 6

NOXA is a direct target of miR-197. (a) Predicted NOXA 3′UTR-binding site for miR-197. The alignment of the seed region of miR-197 with NOXA 3′UTR is shown. The sites of target mutagenesis are indicated in red. (b) qRT-PCR showing inverse correlation of NOXA mRNA and miR-197 expression – mean±S.D., **P=0.0032; (c) western blotting showing the upmodulation of NOXA protein on miR-197 neutralization. (d) Luciferase activity in NIH-H460 cells transfected with NOXA UTR wt or mut in combination with a control or miR-197 mimic. The ratio of normalized luciferase activity in cells transfected with NOXA UTR wt versus mut is indicated – mean±S.E.M., **P=0.0031. Downmodulation of NOXA by RNA interference can rescue LNA-197 apoptotic effect in NIH-H460 cells; shown is Cell Titer-Glo assay (e) and colony formation assay (f) – mean±S.D. **P=0.0086, ***P<0.0001. (g) Downmodulation of p53, NOXA and BMF by RNA interference completely rescues LNA-197 apoptotic effect in NIH-H460 cells as shown by Cell Titer-Glo assay – mean±S.D., ***P<0.0001