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Substrate, sulfate, and headspace amendments for hydrocarbon degrading incubations.
| Axenic or co-culture incubations | Substrate | Sulfate (mM) | Headspace atmosphere |
|---|---|---|---|
| D. alkanexedens ALDC | 1 ml n-decane | 20 | 138 kPa N2/CO2 (80:20) |
| M. hungatei JF-1 | H2/CO2 (138 kPa in ~40 mls of headspace) | – | 138 kPa H2/CO2 (5:95) |
| D. alkanexedens ALDC + M. hungatei JF-1 | 1 ml n-decane | – | 138 kPa N2/CO2(80:20) |
| Uninoculated medium control | 1 ml n-decane | 20 | 138 kPa H2/CO2(80:20) |
“–”, No amendment.
