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. 2014 Apr 1;5:126. doi: 10.3389/fphys.2014.00126

Figure 4.

Figure 4

γ PKC, but not SCA14 mutant γ PKC, mediates the TPA-induced perinuclear translocation of phosphorylated MARCKS. (A) Perinuclear translocation of phosphorylated MARCKS (p-MARCKS) 30 min after TPA treatment. Representative images (γPKC-GFP, p-MARCKS and merged) are shown. Cells expressing γPKC-GFP were treated with TPA for 30 min, followed by immunostaining with an anti-p-MARCKS antibody. The far right image shows the high magnification image of the boxed area in the merged image. p-MARCKS was strongly colocalized with the perinuclear γPKC-GFP, especially around the vesicles. The scale bars of the merged and high magnification images are 5 and 2 μm, respectively. (B) Localization of p-MARCKS in cells expressing GFP alone, WT and S119P γPKC-GFP 30 min after TPA treatment. Representative images (p-MARCKS and GFP) are shown. As described above, p-MARCKS was colocalized with WT γPKC-GFP in the perinuclear region. In contrast, obvious p-MARCKS immunostaining was not observed in cells expressing GFP alone or mutant γPKC-GFP. Scale bar = 10 μm.