Table 1.
Information on the cRNA probes of the 10 investigated classic cadherins.
| Cadherin (Cdh) | Other name | Type | Length of cDNA (insert) (bp) | Accession no. | cRNA probe synthesis |
|---|---|---|---|---|---|
| Cdh2 | Neural cadherin (Ncdh) | Type I | 3203(2739) | NM_001001615.1 | SalI/T3(XbaI/T7) |
| Cdh4 | Retina cadherin (Rcdh) | Type I | 2954(2742) | NM_001004391.1 | HindIII/T3(XbaI/T7) |
| Cdh6 | Fetal kidney-cadherin (Kcdh) | Type II | 2684(2373) | NM_001001758.1 | SalI/T3(XbaI/T7) |
| Cdh7 | – | Type II | 2920(2358) | NM_204187.2 | SalI/T3(XbaI/T7) |
| Cdh8 | – | Type II | 3676(2400) | NM_001100289.1 | XbaI/Sp6(KpnI/T7) |
| Cdh9 | T1-cadherin | Type II | 3542(2370) | XM_001231540.3 | XbaI/Sp6(KpnI/T7) |
| Cdh11 | Osteoblast cadherin (OB-cdh) | Type II | 5423(2379) | NM_001004371.1 | XbaI/Sp6(KpnI/T7) |
| Cdh12 | N-cadherin-2; Br-cadherin | Type II | 3136(2409) | XM_418999.4 | BamHI/T7(XhoI/Sp6) |
| Cdh18 | Cadherin-14 | Type II | 5273(2372) | XM_426046.4 | XbaI/Sp6(KpnI/T7) |
| Cdh20 | MN-cadherin | Type II | 3172(2397) | NM_204134.1 | XbaI/Sp6(KpnI/T7) |
For example, Cdh2 (also called neural cadherin or Ncdh) belongs to the type I classic cadherins. The total cDNA length is 3203 bp and the length of the insert in the recombinant cloning plasmid is 2739 bp. The reference accession number for the NCBI database is NM_001001615.1. The plasmid containing the Cdh2 insert is linearized with the restricted enzyme SalI and transcribed in vitro into cRNA with RNA polymerase T3 to obtain antisense probe; the information in parentheses is for synthesis of sense probe.