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. 2014 Mar 31;8:17. doi: 10.3389/fnana.2014.00017

Figure 3.

Figure 3

Paired photomicrographs showing retrogradely-labeled tectothalamic cells that are GAD-immunopositive (GAD+) or GAD-negative and that project to the ipsilateral MG (A–C) or to the contralateral MG (D–F). The top row in each pair shows cells retrogradely labeled by red beads (RB), FluoroGold (FG), or Fast Blue (FB). The bottom row in each pair shows the same cells viewed for immunoreactivity to GAD, visualized with AlexaFluor 488 (green). In all panels, arrows indicate cells that were double-labeled with a tracer and the GAD immunostain. Arrowheads indicate cells that were retrogradely labeled but immunonegative for GAD. (A–C) Cells labeled by retrograde transport from the ipsilateral MG. Examples are shown from each IC subdivision. (A) ICc: RB-labeled cell (GP638). (B) ICd: FB-labeled cells (GP638). (C) IClc: FG-labeled cell (GP636). (D–F) Cells labeled by retrograde transport from the contralateral MG. Examples are shown from each IC subdivision. (D) ICc: FG-labeled cells (GP636), (E) ICd: FB-labeled cell (GP640). (F) IClc: RB-labeled cell (GP636). Scale bars = 20 μm. ICc, IC central nucleus; ICd, IC dorsal cortex; IClc, IC lateral cortex.