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In vitro and in situ competitive ABPP of isolated enantiomers of 5. (A) In vitro gel-based competitive ABPP of pure enantiomers of 5 in PC3 cell proteomes showing that (−)-5 is more potent than (+)-5 for inhibiting ABHD10 and ACOT1/2. (B) In situ gel-based competitive ABPP of PC3 cells treated with DMSO or (−)-5 (0.001–10 μM) for 2 h, showing that (−)-5 retains its activity in cells.
