Table 2.
Summary of primers for fungal rDNA amplification used in mycobiome studies
| Amplicon | Primer sets | Primer sequence | Length* | Citation | |
|---|---|---|---|---|---|
| ITS | ITS1 | ITS1F/ ITS2 |
CTTGGTCATTTAGAGGAAGTAA | 260 bp | [8,31] |
| GCTGCGTTCTTCATCGATGC | |||||
| ITS2 | 3271-ITS2F/ 3271-ITS2R |
CARCAAYGGATCTCTTGG | 340-360 bp | [19] | |
| GATATGCTTAAGTTCAGCGGGT | |||||
| ITS1-5.8S-ITS2 | ITS1F/ ITS4 |
CTTGGTCATTTAGAGGAAGTAA | 550 bp | [65] | |
| TCCTCCGCTTATTGATATGC | |||||
| LSU | LROR_F/ LR5-F |
CCGCTGAACTTAAGCATATCAATA | 860 bp | [65] | |
| CGATCGATTTGCACGTCAGA | |||||
| NL1/ NL4 |
GCATATCAATAAGCGGAGGAAAAG | 600 bp | [66] | ||
| GGTCCGTGTTTCAAGACGG | |||||
| SSU | 18S_0067a_deg/NSR 399 | AAGCCATGCATGYCTAAGTATMA | 350 bp | [21] | |
| TCTCAGGCTCCYTCTCCGG | |||||