. 2014 Mar 3;7(3):265–310. doi: 10.3390/ph7030265

Table S1.

Antimicrobial activity of fish AMPs. Bacteria and fungi included MICs (μM, or μg/mL will be indicated) values, MLCs (μM), vLD₉₀ (virtual 90% lethal dose, mg/mL [mean ± SEM]) or IC₅₀ (μM). If values are not included antimicrobial activity was assessed by inhibitory halo (IH) and size in millimeter was not presented, if so, is indicated. If isoforms were tested, the best performance value was used. For parasites, the minimum protozoacidal concentration (PC_min) or MIC is indicated. Habitat of microorganisms are indicated (freshwater, marine or other).

Bacteria	β-Defensin	Piscidin	Hepcdin	Cathelicidin	Histone-derived	Pathogenic		Habitat
Bacteria	β-Defensin	Piscidin	Hepcdin	Cathelicidin	Histone-derived	Fish	Human	Habitat
Gram negative
Escherichia coli	39.0 ± 3.7 (vLD₉₀) [60] 32.6 ± 1.5 (vLD₉₀) [52] IH [10]	5–10 μM (MIC) [50] 2–10 μM (MIC) [117] 50 μg/mL (MIC) [40] 2.2–3.3 μM (MIC) [6] 5 μM (MIC) [43] 3–6 μM (MIC) [47] 2 μg/mL (MIC) [83] 3 μM (MIC) [75] 1 μg/mL (MIC) [8]	5–10 μM (MIC) [23] >96 μM (MIC) [62] 6-12 μM (MIC) [171] 18.66 (IC₅₀) [36] 11 μM (MIC) [161] 12–24 μM (MIC) [48] >8.18 μM (MIC) [37] 34.56 mm² (IH) [49]	<1 μg/mL (MIC) [187] 1 μg/mL (MIC) [225] [20] 2–4 μg/mL (MIC) [77] 5 μg/mL (MIC) [186]	1 μg/mL(MIC) [29] >10 μg/mL(MIC) [14] 2.5 μg/mL (MIC) [38]	X	X	Freshwater and others
Plesiomonas shigelloides			11 μM (MIC) [161]			X	X	Freshwater
Klebsiella pneumoniae		12.6 μg/mL (MIC) [82] 2.5–5 μM (MIC) [41]	22 μM (MIC) [161]	18.8 μM (MIC) [77]			X	Other
Klebsiella oxytoca		5–10 μM (MIC) [41] 12.5 μg/mL (MIC) [58]	>44 μM (MIC) [161]				X	Other
Shigella sonnei		5–10 μM (MIC) [41]	>44 μM (MIC) [161]				X	Other
Shigella flexneri		3.1 μg/mL (MIC) [40] 2.5–5 μM (MIC) [41]	>96 μM (MIC) [62] 22 μM (MIC) [161]				X	Freshwater and others
Yersinia enterocolitica		2.5–5 μM (MIC) [41] 100 μM (MIC) [58]	22 μM (MIC) [161]			X	X	Freshwater and others
Yersinia ruckeri		20–40 μM (MIC) [50]				X		Freshwater
Aeromonas salmonicida	>50 μM (MIC) [11]	17.7–35 μM (MIC) [6] 2 μg/mL (MIC) [83] 1 μg/mL (MIC) [8] 5 μM (MLC) [71]	>44 μM (MIC) [161]	9.4 μg/mL (MIC)[77] 1–5 μg/mL (MIC) [225] 50 μg/mL (MIC) [20] 10 μg/mL (MIC) [186]	20 μg/mL (MIC) [38] >1.2 μg/mL (MIC) [66]	X		Freshwater and marine
Aeromonas hydrophila	13.4 ± 0.7 [52] IH [51]	1.05 μg/mL (MIC) [78] 19.78 μg/mL (MIC) [78] >21.4 μg/mL (MIC) [78] >160 [50] >20 [41] >96 [47]	10–20 μM (MIC) [23] >96 μM (MIC) [62] 1.5–3 μM (MIC) [171] >44 μM (MIC) [161] 3–6 μM (MIC) [48] 31.66 mm² (IH) [49]			X	X	Freshwater and others
Aeromonas sobria	59.4 ± 8.8 [60]	10–20 μM (MIC) [50]			2.5 μM (MIC) [38]	X	X	Freshwater and others
Aeromonas punctata		10–20 μM (MIC) [50]				X		Freshwater and others
Vibrio anguillarum	>50 μM (MIC) [11] 58.2 ± 23.4 (vLD₉₀) [60] 21.1 ± 1 (vLD₉₀) [52]	20–40 μM (MIC) [50] 6.3 μg/mL (MIC) [40] 1.25 μM (MLC) [71]	2.92 μM (MIC) [37]	5 μM (MIC) [187] 0.5–2.5 μM (MIC) [225] 5 μM (MIC) [186]		X		Marine
Vibrio parahaemolyticus			>60 μM (MIC) [23] >96 μM (MIC) [62] >48 μM (MIC) [171] 3–6 μM (MIC) [48] 5.84 μM (MIC) [37] 94.25 mm² (IH) [49]	25 [20]		X	X	Marine
Vibrio fluvialis	43.0 ± 10 (vLD₉₀) [60] 35.1 ± 2.7 (vLD₉₀) [52]		>96 μM (MIC) [62] 123.11 mm² (IH) [49]	3.1 μg/mL (MIC) [20]		X	X	Marine
Vibrio harveyi		12.5 μg/mL (MIC) [58] 5 μM (MLC) [71]	20–40 μM (MIC) [23] >96 μM (MIC) [62] 6–12 μM (MIC) [48] 5.84 μM (MIC) [37] 113.10 mm² (IH) [49]	6.2 μg/mL (MIC) [20]		X		Marine
Vibrio alginolyticus		0.03 μg/mL (MIC) [78] 1.24 μg/mL (MIC) [78] 2.68 μg/mL (MIC) [78]	>60 μM (MIC) [23] >96 μM (MIC) [62] >48 μM (MIC) [171] 12–24 μM (MIC) [48] 118.06 mm² (IH) [49]			X	X	Marine
Vibrio vulnificus		0.03 μg/mL (MIC) [78] 0.62 μg/mL (MIC) [78] 0.67 μg/mL (MIC) [78] 6.25 μg/mL (MIC) [58] 2.5 μM (MLC) [71]	20 μM (MIC) [61,163]			X	X	Marine
Vibrio cholera		2.5–5 μM (MIC) [41]				X	X	Freshwater, marine and others
Vibrio damsela		48 μM (MIC) [75]				X	X	Marine
Vibrio penaeicida		5 μM (MLC) [71]						Marine
Salinivibrio costicola		3.125 μg/mL (MIC) [58]						Marine and others
Edwardsiella tarda			>44 μM (MIC) [161] 2.92 μM (MIC) [37]			X	X	Marine and freshwater
Riemerella anatipestifer		6.25 μg/mL (MIC) [114]	25 μg/mL (MIC) [114]					Other
Pseudomonas aeruginosa	44.5 ± 11.8 [60]	0.52 μg/mL (MIC) [78] >19.78 μg/mL (MIC) [78] 10.70 μg/mL (MIC) [78] 60 μg/mL (MIC) [58] 1 μg/mL (MIC) [83] 28 μg/mL (MIC) [82] >35 μM (MIC) [6] 5–10 μM (MIC) [41]	>44 μM (MIC) [161] >96 μM (MIC) [62]	12.5 μg/mL (MIC) [20] 1–4 μg/mL (MIC) [77]		X	X	Freshwater, marine and others
Pseudomonas fluorescens		10–20 μM (MIC) [50] 1.5–3 μM (MIC) [47]	24–48 μM (MIC) [62]		20 μM (MIC) [38]	X	X	Freshwater and others
Pseudomonas stutzeri			<1.5 μM (MIC) [62] 3–6 μM (MIC) [171]				X	Other
Enterobacter cloacae		10–20 μM (MIC) [41] 100 μg/mL (MIC) [58]	>44 μM (MIC) [161]			X	X	Freshwater and others
Enterobacter aerogenes		10–20 μM (MIC) [41] 25 μg/mL (MIC) [58]					X	Others
Salmonella arizonae		10–20 μM (MIC) [41]	>44 μM (MIC) [161]				X	Other
Salmonella choleraesuis		10–20 μM (MIC) [41] 6 μM (MIC) [75]	>44 μM (MIC) [161]				X	Other
Salmonella typhimurium		8.8–17.7 μM (MIC) [6] 10–20 μM (MIC) [41] 2 μg/mL (MIC) [83] 1 μg/mL (MIC) [8]	>44 μM (MIC) [161]		2 μM (MIC) [29]		X	Other
Serratia marcescens		>35 μM (MIC) [6] >20 μM (MIC) [41]	>44 μM (MIC) [161]		4 μM (MIC) [29]		X	Other
Cytophaga columnare		40–80 μM (MIC) [50]				X		Freshwater
Cytophaga aquatilis		2.2–4.4 μM (MIC) [6]				X		Freshwater
Proteus vulgaris		2–10 μM (MIC) [117]X				X	X	Freshwater and others
Photobacterium damsela subsp. piscidida		1.5 μg/mL (MIC) [40]				X		Marine and freshwater
Pasteurella haemolytica		4.4–8.8 μM (MIC) [6]					X	Other
Burkholderia cepacia		>20 μM (MIC) [41]					X	Freshwater and others
Moraxella catarrhalis		2.5–5 μM (MIC) [41]					X	Other
Neisseria gonorrhoeae		>20 μM (MIC) [41]					X	Other
Psychrobacter sp.		10 μM (MIC) [43]						Marine and others
Morganella morganii		12 μM (MIC) [75]					X	Other
Enterococcus faecium		15 μM (MIC) [75]					X	Other
Gram positive
Micrococcus luteus	25–50 μM (MIC) [11] 296.5 ± 65.5 (vLD₉₀) [60] 311 ± 15.6 (vLD₉₀) [52]	10–20 μM (MIC) [41] 3.125 μg/mL (MIC) [58]	>96 μM (MIC) [62] 1.5–3 μM (MIC) [48] 2.5–5 μM (MIC) [23] 24–48 μM (MIC) [62] 34.56 mm² (IH) [49]				X	Freshwater and others
Staphylococcus aureus	358.5 ± 46.5 (vLD₉₀) [60] 229.8 ± 12.8 (vLD₉₀) [52] IH [51]	0–2 μM (MIC) [117] 3.1 μg/mL (MIC) [40] 17.7–35 μM (MIC) [6] 62.5 μg/mL (MIC) [82] 1.25–2.5 μM (MIC) [41] 6–12 μM (MIC) [47] 6.25 μg/mL (MIC) [58] 8 μg/mL (MIC) [83] 1.5 μM (MIC) [75]	1.25–2.5 μM (MIC) [53] 1.5–3 μM (MIC) [62] 1.5–3 μM (MIC) [171] >44 μM (MIC) [161] 3–6 μM (MIC) [48] 1.25–2.5 μM (MIC) [23] >8.18 μM (MIC) [37] 47.12 mm² (IH) [49]	11–45 μM (MIC) [77]	2μM (MIC) [29]		X	Other
Staphylococcus epidermidis		5–10 μM (MIC) [41] 12.5 μg/mL (MIC) [58] 8 μg/mL (MIC) [83] 4 μg/mL (MIC) [8]	20–40 μM (MIC) [23] >96 μM (MIC) [62] 6–12 μM (MIC) [48]		10 μM (MIC) [38]		X	Other
Staphylococcus saprophiticus		5–10 μM (MIC) [41] 7.5 μM (MIC) [75]					X	Other
Staphylococcus haemolyticus		7.5 μM (MIC) [75]					X	Other
Staphylococcus xylosus		>20 μM (MIC) [41] 50 μg/mL (MIC) [58]						Other
Staphylococcus warneri		15 μM (MIC) [75]						Other
Bacillus subtilis		1.1–2.2 μM (MIC) [6] 0.75–1.5 μM (MIC) [47] 48 μM (MIC) [75]	5–10 μM (MIC) [23] >96 μM (MIC) [62] 11.41 (IC₅₀) [36] 3–6 μM (MIC) [48] 28.86 mm² (IH) [49]		11 μg/mL (MIC) [29] 0.6 μg/mL (MIC) [66] 1.3 μg/mL (MIC) [38]			Other
Bacillus cereus	17.5 ± 4 (vLD₉₀) [60]	0–2 μM (MIC) [117] 5 μM (MIC) [43]	40–60 μM (MIC) [23] >96 μM (MIC) [62] 12–24 μM (MIC) [48]				X	Other
Corynebacterium glutamicum			2.5–5 μM (MIC) [23] 48–96 μM (MIC) [62] 3–6 μM (MIC) [171] 1.5–3 μM (MIC) [48]					Other
Planococcus citreus	0.4–0.8 μM (MIC) [11]				0.08 μg/mL (MIC) [66]			Marine
Enterococcus faecalis		8.39 μg/mL (MIC) [78]	>44 μM (MIC) [161]				X	Other
		9.89 μg/mL (MIC) [78]
		>21.41 μg/mL (MIC) [78]
		3.1 μg/mL (MIC) [40]
		>256 μM (MIC) [210]
		2.5–5 μM (MIC) [41]
		8-16 μg/mL (MIC) [209]
Listeria monocytogenes		2.5–5 μM (MIC) [41] 25 μg/mL (MIC) [58]					X	Other
Streptococcus agalactiae		0.13 μg/mL (MIC) [78]				X	X	Freshwater, marine and others
		0.31 μg/mL (MIC) [78]
		0.33 μg/mL (MIC) [78]
		1.25–2.5 μM (MIC) [41]
		12.5 μg/mL (MIC) [58]
Streptococcus iniae		3.1 μg/mL (MIC) [40] 1.25–2.5 μM (MIC) [41] 1.5 μM (MLC) [71]				X	X	Freshwater, marine and others
Streptococcus mutans		2.2 μM (MIC) [207] 8 μg/mL (MIC) [210] 4 μg/mL (MIC) [209]			1 μg/mL (MIC) [29]		X	Other
Streptococcus sobrinus		8 μg/mL (MIC) [210] 4 μg/mL (MIC) [209]					X	Other
Streptococcus sanguinis		32 μg/mL (MIC) [210] 4–8 μg/mL (MIC) [209]					X	Other
Streptococcus gordonii		8 μg/mL (MIC) [210] 8 μg/mL (MIC) [209]					X	Other
Streptococcus bovis		1.25–2.5 μM (MIC) [41]					X	Other
Streptococcus equisimilis		2.5–5 μM (MIC) [41]					X	Other
Streptococcus mitis		1.25–2.5 μM (MIC) [41]					X	Other
Streptococcus pneumoniae		1.25–2.5 μM (MIC) [41] 12.5 μg/mL (MIC) [58]					X	Other
Streptococcus pyogenes		1.25–2.5 μM (MIC) [41] 25 μg/mL (MIC) [58]					X	Other
Lactococcus garviae		3.1 μg/mL (MIC) [40] 5 μM (MLC) [71]				X	X	Marine and others
Leucothrix mucor		>35 μM (MIC) [6]				X		Marine
Lactobacillus acidophilus		128 μg/mL (MIC) [210] 4–8 μg/mL (MIC) [209]						Other
Lactobacillus casei		32 μg/mL (MIC) [210] 4 μg/mL (MIC) [209]						Other
Lactobacillus fermenti		2 μg/mL (MIC) [210] 4 μg/mL (MIC) [209]						Other
Actinomyces viscosus		8 μg/mL (MIC) [209]					X	Other
Actinomyces naeslundii		8 μg/mL (MIC) [209]					X	Other
Fungi
Aspergillus fumigatus		50–100 μM (MIC) [41]					X	Other
Aspergillus niger		48–96 μM (MIC) [47]	20–40 μM (MIC) [23] 44 μM (MIC) [161] 12–24 μM (MIC) [48]				X	Other
Fusarium graminearum			20–40 μM (MIC) [23] 12–24 μM (MIC) [48]					Other
Fusarium solani			20–40 μM (MIC) [23] 12–24 μM (MIC) [48]				X	Other
Fusarium oxysporum		0.78–1.56 μM (MIC) [41]				X	X	Freshwater, marine and others
Fusarium culmorum		0.39–0.78 [41]						Other
Candida albicans		15.4 μg/mL (MIC) [82] 10–20 μM (MIC) [41] 24–48 μM (MIC) [47] 8 μg/mL (MIC) [83] 96 μM (MIC) [75] 4 μg/mL (MIC) [8] 5 μM (MIC) [115] 6.25 μM (MIC) [226]	>60 μM (MIC) [23] >96 μM (MIC) [62] >44 μM (MIC) [161] >48 μM (MIC) [48]	2.3 [77] 2.5 [186]			X	Other
Candida glabrata		10–20 μM (MIC) [41]					X	Other
Candida lusitania		10–20 μM (MIC) [41]
Candida tropicalis		10–20 μM (MIC) [41]					X	Other
Saccharomyces cerevisiae		384 μM (MIC) [75] 5 μM (MIC) [115]					X	Other
Pichia pastoris			>60 μM (MIC) [23] >96 μM (MIC) [62] >48 μM (MIC) [48]					Other
Saprolegnia sp		12.5–25 μg/mL (MOC) [98]				X		Freshwater and others
Neurospora crassa		1.56–3.12 μM (MIC) [41]						Other
Trichosporon beigelii		2.5 μM (MIC) [115] 1.56 μM (MIC) [226]					X	Other
Malassezia furfur		6.25 μM (MIC) [226]					X	Other
Parasites
Trichomonas vaginalis		12.5 μg/mL (MIC) [115]					X	Other
Trichodina		12.5–100 μg/mL (PC_min) [97]				X		Freshwater and marine
Cryptocaryon theront		12.5 μg/mL (PC_min) [97]				X		Marine
Amyloodinium dinospore		6.3 μg/mL (PC_min) [97]				X		Marine
Ichthyophthirius theront		6.3 μg/mL (PC_min) [97]				X		Freshwater
Virus
VHSV	[64]		[80]			X		Marine
NNV	[59]	[227]	[99,169]			X		Marine
IPNV			[170]			X		Marine
RGV	[60]					X		Marine and Freshwater
SGIV	[59]		[61]			X		Marine
CCV		[100]				X		Freshwater
FV3		[100]						Other