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. 2014 Apr 8;4:4423. doi: 10.1038/srep04423

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Left panel: MSAP amplicon produced with the HM5-E36 primer combination. 1 indicates samples obtained in 2003. 2 indicates samples obtained in 2007. M indicates digestion with MspI. H indicates digestion with HpaII (e.g., sample T1M corresponds to genotype Tanganyika, year 2003, MspI digestion). Different cytosine methylation patterns are indicated by arrows on the left. Right panels: AFLP amplicons produced with the M38-E33 and M38-E36 primer combinations. 1 indicates samples obtained in 2003. 2 indicates samples obtained in 2007 (e.g., sample T1 corresponds to genotype Tanganyika, year 2003). Different genetic patterns produced by primer combination M38-E36 are indicated by arrows on the left. Full length blots are presented in the Supplementary Information.