Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 23;16(1):R9. doi: 10.1186/bcr3601

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Rexer et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

BT474 lapatinib-resistant cells acquire E542K PIK3CA mutation. (A) SNaPshot analysis of genomic DNA isolated from parental and lapatinib-resistant (LR) cells reveals a distinct peak corresponding to a G1624A nucleotide change in resistant cells. (B) Sanger sequencing of exon 9 of PIK3CA confirms the presence of the G1624A nucleotide change in LR cells. (C) Lysates from parental and LR cells treated with increasing doses of lapatinib, ranging from 0.001 to 10 μM, were analyzed by immunoblotting with the indicated antibodies.