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Bright field microscopy images of BAECs cultured on spNIPAM (bottom), ppNIPAM (middle), and blank glass control (top) surfaces. The cells attached and proliferated above the LCST (left) on all surfaces. After the temperature was shifted to 4°C (right), as expected there was no cell release from the control surfaces. There was complete cell detachment from ppNIPAM surfaces as a sheet, but aggregated clumps of cells detached from spNIPAM films. (Black scale bars = 100μm)
