Table 7.
Real-time PCR analysis of 14 selected genes using a different set of donors reveals similar correlation with RNA-Seq results
| Gene name | Differential expression | Age | P value | |
|---|---|---|---|---|
| Young | Old | |||
| DKK1 | Higher in old | 0.0004 ± 0.0006 | 0.008 ± 0.0006 | 0.04 |
| COL10 | Higher in old | 7.66 × 10-5 ± 3.05 × 10-5 | 0.000148 ± 7.13 × 10-5 | 0.1 |
| RUNX2 | Lower in old | 0.002 ± 0.0004 | 0.0005 ± 0.0004 | 0.02 |
| SRPX | Lower in old | 0.0025 ± 0.0002 | 0.001 ± 0.002 | 0.05 |
| ACSL5 | Lower in old | 0.004 ± 0.002 | 0.002 ± 0.02 | 0.13 |
| IL7R | Lower in old | 0.001 ± 0.0002 | 0.0007 ± 0.0004 | 0.07 |
| COL2A1 | Lower in old | 32.6 ± 24.5 | 0.2 ± 0.13 | 0.04 |
| COL1A1 | Lower in old | 0.71 ± 0.11 | 0.02 ± 0.02 | 0.0003 |
| MMP1 | Lower in old | 0.28 ± 0.17 | 0.07 ± 0.03 | 0.03 |
| MMP13 | Lower in old | 0.08 ± 0.12 | 0.02 ± 0.02 | 0.28 |
| ADAMTS-4 | Lower in old | 0.07 ± 0.05 | 0.03 ± 0.02 | 0.07 |
| IL1β | Lower in old | 0.0001 ± 0.0002 | 2.66 × 10-5 ± 1.14 × 10-5 | 0.03 |
| TNFα | No change | 0.0001 ± 7.7 × 10-5 | 9.8 × 10-5 ± 4.5 × 10-5 | 0.4 |
| TBFβ | No change | 0.58 ± 0.14 | 1.06 ± 0.5 | 0.12 |
Values for real-time polymerase chain reaction (RT-PCR) are the mean ± standard deviation of relative expression levels normalised to expression of glyceraldehyde-3-phosphate dehydrogenase. Statistical significance was tested using Student's t test. DKK1, dickkopf homolog 1; COL10, collagen type X; RUNX2, Runt-related transcription factor 2; SRPX, Sushi repeat-containing protein; ACSL5, acyl-CoA synthetase long-chain family member 5; IL7R, interleukin 7 receptor; COL2A1, collagen type II, alpha 1; COL1A1, collagen type I, alpha 1; MMP1, matrix metalloproteinase 1; MMP-13, matrix metalloproteinase 13; ADAMTS4, a disintegrin and metalloproteinase with thrombospondin motifs 4; IL-1β, interleukin 1 beta; TNFα, tumour necrosis factor alpha; TGFβ, transforming growth factor beta.