Table 2.
Targeted Q-PCR analysis of mammary glands after 3, 4, or 10 weeks and mammary tumors on HFD versus LFD
|
Growth factor genes |
Fold change HFD versus LFD |
||||
|---|---|---|---|---|---|
| Symbol | Description | 3 wks MG | 4 wks MG | 10 wks MG | Tumor |
|
Fgf18 |
Fibroblast growth factor 18 |
2.0 |
- |
- |
- |
|
Il4 |
Interleukin 4 |
2.0 |
- |
- |
- |
|
Bdnf |
Brain derived neurotrophic factor |
- |
2.4 |
- |
- |
|
Lefty2 |
Left-right determination factor 2 |
- |
-2.5 |
- |
- |
|
Lif |
Leukemia inhibitory factor |
- |
2.8 |
- |
- |
|
RANKL |
Receptor activator of nuclear factor kappa-B ligand |
- |
17 |
- |
- |
|
Bmp2 |
Bone morphogenetic protein 2 |
- |
- |
2.0 |
- |
|
Bmp3 |
Bone morphogenetic protein 3 |
- |
- |
2.4 |
- |
|
Fgf10 |
Fibroblast growth factor 10 |
- |
- |
2.0 |
- |
|
Gdf10 |
Growth differentiation factor 10 |
- |
- |
1.9 |
- |
|
Gdf5 |
Growth differentiation factor 5 |
- |
- |
2.2 |
- |
|
Nodal |
Nodal |
- |
- |
2.4 |
- |
|
Pgf |
Placental growth factor |
- |
- |
2.5 |
- |
|
Tgfa |
Transforming growth factor alpha |
- |
- |
1.9 |
- |
|
Tgfb1 |
Transforming growth factor, beta 1 |
- |
- |
1.9 |
- |
|
Vegfa |
Vascular endothelial growth factor A |
- |
- |
2.1 |
- |
|
Il1a |
Interleukin 1 alpha |
- |
- |
3.0 |
- |
|
Il1b |
Interleukin 1 beta |
- |
- |
2.1 |
- |
|
Il2 |
Interleukin 2 |
- |
- |
3.0 |
- |
|
Il7 |
Interleukin 7 |
- |
- |
3.4 |
- |
|
Cxcl12 |
Chemokine (C-X-C motif) ligand 12 |
- |
- |
1.8 |
- |
|
Bmp7 |
Bone morphogenetic protein 7 |
- |
- |
- |
3.2 |
|
Inha |
Inhibin alpha |
- |
- |
- |
2.2 |
|
Ntf3 |
Neurotrophin 3 |
- |
- |
- |
53.4 |
| Bmp10 | Bone morphogenetic protein 10 | - | - | - | -3.4 |
N = 3 high fat diet (HFD) mice, n = 3 low fat diet (LFD) mice, n = 3 HFD mice with early developing tumors (HFD-E), n = 3 LFD mice with tumors. P ≤0.05 for all genes listed.