Skip to main content
. Author manuscript; available in PMC: 2015 Mar 5.
Published in final edited form as: Neuron. 2014 Mar 5;81(5):1009–1023. doi: 10.1016/j.neuron.2014.01.013

Figure 4. Adult SOD1-G93A microglia induce MN death in an NF-κB dependent mechanism in vitro.

Figure 4

(A and B) Representative microscopic fields (A) and well counts (B) of Hb9-GFP+ MNs after 1 day (24 hours) and 3 days (72 hours) in co-culture with WT or SOD1-G93A microglia not infected (black bars), infected with Ad-RFP (dashed bars) or Ad-IκBα-SR (white bars). MNs co-cultured with either WT; IKKβf/f or SOD1-G93A; IKKβf/f microglia infected with Ad-cre shown with dashed bars. Scale bar = 200 microns

(C and D) Quantification of TNF-α (C) and nitric oxide (D) in the co-culture medium by ELISA.

(E) Quantification of phospho-p65 by ELISA from microglial-MN co-cultures. Phospho-p65 normalized to total p65 determined by ELISA.

(F) Luciferase assay of NF-κB activity in microglia. Firefly luciferase normalized to renilla luciferase.

Error bars represent s.e.m. ****, P< 0.0001, ***, P< 0.001. See also Movie S1, Movie S2, Movie S3, and Movie S4.