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Influence of PLA-flurbiprofen nanoparticles on the integrity of the endothelial cell barrier. bEnd.3 cells were cultivated on cell culture inserts in a cellZscope® device. When cells were post-confluent, approximately 2.4 mg nanoparticles per cm2 (corresponding to 750 μM nanoparticulate flurbiprofen) were added to the luminal side of the endothelial cells and the TER was measured every hour by impedance spectroscopy. PLA, polylactide; TER, transepithelial electrical resistance.
